The pulmonary and hepatic hydatid cyst fluids were collected from 540 slaughtered camels and 5 human cases in Qalyubia Governorate, Egypt. The prevalence of infection of cystic echinococcosis among camels was 120 (22.2%). The fertility rates of the isolated cysts form camels and humans were 64.5 and 100%, respectively. A nested polymerase chain reaction was used for amplification of mitochondrial NADH 1 gene of Echinococcus granulosus complex in fertile cysts obtained from camels and humans, respectively. Two pairs of primers (EGL1 and EGR2) and (EGL3 and EGR4) were used in 2 amplification steps. First, the outer pair of primer originated from a highly conserve region of NADH1 gene generate a primary 435 bp PCR product. Second, a pair of internal (nested) primer (EGL3 and EGR4), designed to the annealing site of primers (EGL1 and EGR2) yield similar diagnostic amplified DNA bands of molecular size marker at 276 bp in all examined cysts obtained from camels and humans indicating a zoonotic relationship. This study confirms similar fingerprinting patterns of Echinococcus granulosus complex in camels and humans in Qalyubia Governorate, Egypt. Nested PCR for diagnosis of E. granulosis had been used for the first time in Egypt, as far as we know.
|
