This study was designed to evaluate the protective and detoxificated effect ojascorbic acid (vit. C) against mercuric chloride toxicity, in normal adult albino rats. The experiment was carried out on 60 adult rats weighing between 200 300 g, and divided into 6 groups, each containing 10 rats. The first group acts as a control group. Vitamin C was given to the rats oj the second group in a dose qf 45 mg/LOOg body weight. In the third group, mercuric chloride was given in a dose oj 1 mg /100 g body weight. In theJourth group, Vit C was administrated one hour aJter mercuric chloride injection. While in thefifth group. it was given one hour beJore mercuric chloride. The rats ojthe sixth group. were treated with vitamin C daily Jor one week beJore mercuric chloride administration. Blood
Jor biochemical analysis was collected beJore killing the rats, then animals were killed by cervical dislocation. Liver and kidney were dissected and preparedJor histological and histochemical examination. There was a statistically very highly significant increase in both liver and kidney Junction tests oj mercuric chloride treated rats Jrom the corresponding control group. This also. was confirmed by histological and histochemical changes in the liver and kidney. However, in rats were given ascorbic acid before mercuric chloride, we obsenJed that the biochemical data did not differ
significantly from the control values and also, the histological and histochemical changes were more or less similar to the control. Thus. indicating the protective role oj ascorbic acid against mercuric chloride poisoning. |
