Different Phages parasitizing Xanthomonas vesicatoria (the causal agent of the bacterial spot disease of tomato) were isolated from infected leaves of tomato and from tomato rhizosphere soil, using enrichment technique. The phages produced plaques (4-5mm, diameter) with a distinct translucent spreading halo. Presumptive phage particles associated with X. vesicatoria were observed by Transmission Electron Microscope (TEM). Particle size and morphology of each phage isolate were examined by electron microscopy. The obtained results indicated that, the isolated phages were of the head and tail types. Four phages were detected and designated A,B,C and D, their head diameters were found to be 57.83, 43.84, 63.43, and 79.25 nm, respectively. Phages A and B were isolated from tomato leaves, whereas, phages C and D were isolated from tomato rhizosphere soils. This study seems to be the first record for phages of Xanthomonas vesicatoria under the Egyptian conditions. |
