Seeds of broad bean, varieties Giza 2 and Rebaye 40 were inoculated with potent antagonistic rhizospheric isolates belonging to Bacillus sp. Streptomyces sp. or Trichoderma sp., which proved in earlier experiments
(in vitro) to be potent antagonists against F. solani. R. solani and Scl. rolfsii. Seed inoculation treatments decreased the pre- and post-emergence damping-off and increased the survival plants percentages and decreased the deleterious effect of the pathogens on the growth of survival plants as compared to their respective control (uninoculated seeds). Seed inoculation with the three potent antagonists (Bacillus sp. + Streptomyces sp. + Trichoderma sp. isolates) was the most efficient treatment in controlling the aforementioned pathogens in soil. Multiple antagonists treatment gave lower pre- and post-emergence damping-off and higher survival plants percentages and less deleterious effect of the pathogens on the growth of survival plants than when any of the antagonists was added solely. These results were found in both broad bean varieties Giza 2 and Rebaya 40 sown in sterilized and unsterilized soil infested with the pathogens F. solani, R. solani and Scl. rolfsii. |
