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Dr. Nevien Mohamed Ahmed Gad :: Publications:

Role of Western Blotting in Serological Diagnosis of Helicobacter pylori Infection
Authors: Niveen M.A.Gad , Taghrid Gamal El-Din , Reem R.Abd-El Glil, and *Mohammed Darwish
Year: 2008
Keywords: Not Available
Journal: Egyptian Journal of Medical Microbiology
Volume: VOL. 17
Issue: Not Available
Pages: Not Available
Publisher: Not Available
Local/International: International
Paper Link: Not Available
Full paper Nevien Mohamed Ahmed Gad_H.pylori.pdf
Supplementary materials Not Available

Background: Helicobacter pylori (H.pylori ) is the key pathogen for gastroduodenal diseases. The clinical outcome of H.pylori infection is influenced by the presence of strain-specific virulence factors that are usually detected by the presence of specific anti-H.pylori antibodies in serum. Apart from the detection of these antibodies by enzyme-linked immunosorbent assay (ELISA), it is desirable to obtain additional information concerning the presence of certain virulence factors of H.pylori that could be detected by immunoblot analysis. Objective: the aim of this work was to evaluate if blotting can replace the need for invasive endoscopy for diagnosis of virulent H.pylori infection , compare between it and ELISA as serodiagnostic test, and to focus on identifying factors and markers that define high-risk patients in whom H.pylori infection needs to be eradicated. Subjects and Methods: 19 dyspeptic patients were subjected to upper gastrointestinal endoscopy to obtain antral biopsy, direct urease test and culture of biopsy on specific media . Sera were obtained from the patients for IgG examination by ELISA and western blotting. Results: Western blotting was more sensitive(100%) than ELISA (sensitivity 81.8%),but specificity was the same for both (87.5%).Only western blotting was able to detect antibodies to virulence antigens especially cytotoxin associated antigen(CagA) and vacuolating cytotoxin antigen (VacA).Conclusion : Western blotting is a highly sensitive noninvasive test to diagnose toxigenic H.pylori infection. So that unnecessary gastroscopy and treatment can be avoided.

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