You are in:Home/Publications/REPRODUCIBILITY, STABILITY AND DISCRIMINATORY POWER OF RESTRICTED ENZYME ANALYSIS OF PLASMID DNA AMONG METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS

Dr. Nevien Mohamed Ahmed Gad :: Publications:

Title:
REPRODUCIBILITY, STABILITY AND DISCRIMINATORY POWER OF RESTRICTED ENZYME ANALYSIS OF PLASMID DNA AMONG METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS
Authors: Reem R.Abd El-Glil, Taghrid Gamal El-Din . Niveen M.A. Gad,
Year: 2005
Keywords: Not Available
Journal: AAMJ
Volume: vol 3
Issue: Not Available
Pages: Not Available
Publisher: Not Available
Local/International: International
Paper Link: Not Available
Full paper Nevien Mohamed Ahmed Gad_MRSA.pdf
Supplementary materials Not Available
Abstract:

Methicillin-resistant Staphylococcus aureus (MRSA) is a significant epidemiological problem. Detecting the sources of epidemic strains and preventing their access to patients, however, depend upon the availability of techniques to reliably distinguish among MRSA strains. Molecular typing of MRSA generally demonstrates improved discriminatory power compared with assays of phenotypic traits, and it is frequently preferred. Objective :Evaluation of the discriminatory power and stability of restricted enzyme analysis of plasmid (REAP) DNA among MRSA strains. Material and Methods: 54 sequential MRSA isolates from 18 patients collected within 30-36 days ( Three isolate from every patient , in average 15 days interval between each isolate) .Isolates were subjected to plasmid screening ; isolates with positive plasmid underwent typing by REAP using Hind III restriction enzyme . Comparison were done to detect plasmid stability and discriminatory power among sequential MRSA isolates. Result :Among 54 sequential MRSA isolates; 45 were plasmid- positive isolates from 15 patients by plasmid screening gel . Patient’s plasmid digestion profile remained unchanged over a period up to one month except in two cases (4.4%) . In one case , two distinctly different digestion profiles were detected from a patient ( between the initial isolate and the second successive isolate) .In the other case, two distinctly different digestion profiles were detected from a patient (between the initial isolate and the third successive isolate).Nine (16.6%) out of 54 MRSA isolates from 3 patients lacked plasmid. Conclusion The frequency of genetic alteration in plasmid among MRSA strains in vivo are infrequent and REAP profile of MRSA isolates is very stable in vivo. The diversity and stability of MRSA plasmid types make them excellent epidemiological markers.

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