Endophytic bacterial isolates were isolated from roots, nodules, leaves and
stems of faba bean ( Vicia faba ) , pea ( Pisum sativum ) , fenugreek ( Trigonella foenumgracum
) , lupine ( Lupinus spp.), common bean (Phaseolus vulgaris) and rice (Oryza
sativa) at flowering stage. A total of 167 endophytic isolates were screened qualitative
and quantitative for cellulase and pectinase activities. Result showed that more than 55
isolates out of 167 were able to produce cellulase and pectinase enzymes. Total of 55
isolates were screened as plant growth-promoters (PGP) traits and root colonization. The
highest values of log CFU was 6.4x105 of isolate TN10 inside the roots of faba bean plant.
Moreover, 12 endophytic isolates produced IAA more than 25μ/ml in the presence of the
precursor tryptophan. Also TN12, HN32 and RN62 isolates recorded the highest values of
siderophores production. About 82% of the isolates showed positive results of HCN, 44
isolates were able to produce ammonia. The phosphate solubilization efficiency percentage
were detected for endophytic isolates, 19 isolates showed the maximum range of phosphate
solubilization efficiency (SE). |
