Obesity has been described as a state of chronic oxidative stress and inflammatory reaction. This study was carried out to clarify the effect of exercise and antioxidant quercetin on oxidative stress and inflammatory reaction induced by obesity in renal tissue and to detect the role of adiponectin.Rats were randomly divided into five groups 8 animals each: Group I,received balanced diet; Group II, received high fat diet; Group III,received high fat diet and were subjected to exercise training for the last 8 weeks; Group IV,received high fat diet and treated with quercetin at a dose of 15gm/kg intraperitoneal (IP) daily in the last 8 weeks; Group V,received high fat diet and were subjected to exercise training combined with quercetin treatment in the last 8 weeks. The study was carried out over 28 weeks. Obesity induced by high fat diet (HFD) was confirmed by measuring body weight and body mass index (BMI). Obese rats developed insulin resistance confirmed biochemically by significant increase in fasting blood glucose, fasting insulin and homeostasis model assessment of insulin resistance (HOMA-IR) index. Moreover,there was significant increase in serum urea, serum creatinine,albuminurea, renal malondialdehyde (MDA) and tumor necrosis factor alpha (TNF-α) levels with significant decrease in superoxide dismutase (SOD) ,reduced glutathione (GSH) and adiponectin. Both exercise training for 30-45 minutes /day and quercetin treatment at a dose of 15gm/kg intraperitoneal (IP) daily for 8 weeks were sufficient to cause improvement in insulin resistance, kidney functions and renal oxidative stress evidenced by significant improvement of all the previous parameters, with significant decrease in TNF-α and increase in adiponectin. The most improvement was among combined exercise & antioxidant group. We concluded that ani-oxidative and anti-inflammatory effect of exercise &quercetin may be through modulation of adiponectin. |
