The probable beneficial effects of mesenchymal stem cells (MSCs) and resveratrol were assessed in an experimental model of Bisphenol-A (BPA)-evident uterine damage in rats. Thirty-five albino rats were involved and
equally divided into five groups: Group I: negative control rats received usual diet, Group II: positive control rats
received BPA by oral gavage for 15 days, Group III: BPA-treated rats received single oral gavage of resveratrol
daily for two weeks, Group IV: BPA-treated rats received a single intravenous dose of MSCs and Group V: BPAtreated rats received combined treatment of resveratrol and MSCs. Oxidative stress markers, apoptosis-related
genes, and gonadal hormones were assessed. Histological and immunohistochemical examination of uterine
tissue was conducted for TGF-β 1. Caspases-3, 8, and 9 (Casp3, Casp8, Casp9) genes were assessed in uterine
tissues by quantitative real-time PCR. Results revealed that BPA induced significant changes in the endometrial
tissue, inflammatory cell infiltration, focal blood extravasation, increase in collagen fibers, decrease in PAS
staining, and increase in TGF-β 1 immunoreactivity. BPA also induced a significant increase in oxidative stress
markers; malondialdehyde (MDA), SOD, CAT, and apoptosis-related genes. BPA induced a significant change in
blood levels of gonadal hormones; a significant increase in FSH and a significant decrease in estradiol (E2) and
progesterone (P). Treatment with either resveratrol, MSCs, or a combination of them resulted in significant
enhancement of histological findings, restoration of gonadal hormones to near-normal levels, and a significant
decrease in oxidative stress markers and apoptosis genes. Combined treatment with resveratrol and MSCs
demonstrated more significant therapeutic effects as regard to the studied parameters in association with rat
groups treated with either MSCs or resveratrol separately |
