Bisphenol-A is the building block of polycarbonate plastics, a hard plastic used to make numerous consumer products, including most baby and water bottles. Its final product includes adhesives, coatings, paints and building materials. BPA waste may enter the environment during handling, loading and unloading, heating or releases. BPA can enter the human body through reusable baby bottles, food packing materials, liquid of canned vegetables and dental sealants.
Bisphenol-A (BPA) is one of the most ubiquitous synthetic chemical compounds in production today, with over three million tons produced annually. The extensive use of BPA means that environmental exposure is very common. Recent research indicated that through this everyday exposure BPA acts as an endocrine disrupting compound even at low doses. so most studies are focused on its effect on reproductive organs. Beside its endocrine disrupting effect, BPA can also induce oxidative stress.
Oxidative stress has long been linked with pathogenesis of various diseases in humans. It takes place due to unregulated production of free radicals or reactive oxygen/nitrogen species. Reactive oxygen species (ROS) are cytotoxic agents that lead to damage of nucleic acid bases, lipids and proteins, thereby leading to cell death.
There is limited information considering the toxic effect of BPA on different organs, so that this study was done to demonstrate the effect of BPA on the liver and kidney in albino rats and wheather co-administration of vitamin C could prevent this toxicity or not.
The study was conducted on 72 normal adult albino rats of both sexes divided into 6 groups,
• Group I (negative control group): lifted without intervention to measure the basic parameters.
• Group II (positive control group): Each rat was treated with olive oil (vehicle of bisphenol A & vitamin C) once daily, orally for six weeks.
• Group III (vitamin C treated group): each rat was treated with vitamin C (60mg/kg/day) dissolved in olive oil, once daily, orally, for six weeks.
• Group IV (bisphenol -A treated group): Each rat was treated with bisphenol A (25 mg/kg/day) dissolved in olive oil, once daily, orally, for six weeks.
• Group V (treated with bisphenol A and high dose of vitamin C): Each rat was treated with bisphenol A (25 mg/kg/ day) and vitamin C (60 mg /kg/ day) once daily, orally, for six weeks.
• Group VI (treated with bisphenol A and low dose of vitamin C): Each rat was treated with bisphenol A (25 mg/kg/ day) and vitamin C (5.5 mg /kg/ day) once daily, orally, for six weeks.
Treatment continued for 6weeks. After the end of 6thweek, rats from each group was sacrificed for estimation of:
• Serum AST, ALT, uric acid & creatinine.
• SOD, catalase, GPX & MDA in both hepatic and renal tissues.
• Histopathological examination of hepatic and renal tissues by light & electron microscopy.
The interpretation of the results of the present study revealed that BPA induced hepato and nephrotoxicity by infliction of oxidative stress, as oral administration of bisphenol A had resulted in a significant increase in MDA which is a marker of lipid peroxidation (LPO), and a significant (p |
