Background: Deltamethrin (DLM) is class II synthetic pyrethroid used worldwide
as pesticides in agriculture, household pest control, protection of foodstuff, and disease
vector control. Exposures occur mainly from the household application, contaminated
food or water. Although initially thought to be least toxic, a number of recent studies
revealed its toxic effects in different animal species. Aim: The current study aimed to
evaluate oxidative stress effect due to DLM exposure on brain tissues of adult albino
rats, and whether co-administration of pomegranate (PM) molasses can ameliorate this
oxidative damage. Materials & Methods: Thirty adult experimental Albino rats were
divided into five groups: -ve control group (6 rats), +ve control group (6 rats)
(received 1ml corn oil once daily, orally), diluted PM molasses [(0.5ml (PM)
molasses+(0.5ml) distilled water] treated group (6 rats), DLM (6mg/kg) treated group
(6 rats), DLM (6mg/kg) + diluted PM molasses[(0.5ml (PM) molasses+(0.5ml)
distilled water] treated group (6 rats). These doses were given once daily, orally for
seven days. By the end of the expirement, blood sample were obtained for estimation
of plasma cholinesterase (PCHE) level and oxidative stress parameters )
malondialdehyde [MDA], glutathione [GSH], and catalase [CAT] (, then rats were
sacrificed. The brains were excised, prepared for estimation of oxidative stress
parameters in tissue, histopathological changes and immunohistochemical
examination. Results: revealed that DLM caused neurotoxicity in albino rats as there
was a significant increase in serum & brain tissues MDA, reduction of both GSH &
CAT, decrease in PCHE level & pathological changes in brain included, neuronal
degeneration, apoptotic bodies, neuropil vacuolizations, proliferated and dilated blood
vessels beside strong positive immunreaction for bax when compared with both
control groups & PM molasses treated groups. While, there was significant reduction
in serum & brain MDA, significant increase in both GSH &CAT and also decrease in
PCHE level. Mild pathological changes in brain of albino rats beside mild positive
immunreaction for bax in DLM + PM molasses treated group as compared to DLM
treated group. Conclusion: deltamethrin can induce oxidative damage in the
experimental albino rat brain & pomegranate molasses as an antioxidant has an
ameliorative effect against this damage. |
