This study declared a trial forthe preparation and assessment of Montanide gel 01 adjuvant inactivated Strangles vaccine. This vaccine was prepared from Streptococcus equi subs. equi(field isolate)that was confirmed by PCR. The prepared vaccine was assessed by using two species of animals, horse as a main host and rabbit as a laboratory animal. A total of 10 male horses were equally divided into 2 groups’ first one wasvaccinated with the vaccine, the secondone was control and serum antibodies titer was measured for two groups for six months. Twenty New-Zealand rabbits were used as follow; fifteen rabbits were divided into 2 groups. The first group contains 6 rabbits were used as a vaccinated group which was vaccinated and challenged, the second group 9 rabbits were used as a control was divided into 2 groups (one contains 6 (positive control) were infected intendedly at the challenge time and another containing 3 were negative control). Another five rabbits were used in a purity of vaccine. ELISA results of horse showed high significant value in the vaccinated group than control along the study. ELISA results of rabbit also showed highly significant value in the vaccinated group than the positive control group on along the study except for 2nd and 3rd weeks post challenge. The result of histopathologicalexamination of various sections of submaxillary lymph node of negative control rabbits revealed normal lymphoid follicles While in positive control showed nuclear pyknosis, karyorrhexis or karyolysis, with the presence of empty areas and eosinophilic cellular debris in some other areas. However, the vaccinated group showed marked activation of their germinal centers with the appearance of many tangiblebodies, macrophages, and some apoptotic cells and Mild to moderate degree of lymphocytic degenerative and necrotic changes. Briefly, the prepared vaccine was given good protection. |
