Poly Cystic Ovarian Syndrome (PCOS) is increasingly reported nowadays. Recently exosomes released by
Mesenchymal Stem Cells (MSCs-EX) has been a novel source with a great potential donor cell in regenerative
medicine because of their low immunogenicity and easy accessibility. Foeniculum vulgare (FVE) is a naturally
occurring estrogen compound, which is commonly used today due to the impact of the female hormones.
In this study, we evaluated the therapeutic effect of exosome released by MSCs in experimentally induced Poly
Cystic Ovary (PCO). The polycystic disease was artificially induced by injecting rats daily with testosterone propionate
(dissolved in propylene glycol) 1 mg/100 g body weight for 35 days. Exosomes were prepared for 3rd passage of
MSCs-EX and were injected into the induced PCO rats. Another group of PCOS rats received FVE (alcoholic extract)
150 mg/kg body weight/day intra gastric for five days after induction of PCOS. The ovaries were taken for histological
examination and immune-histochemical detection of Octamer-Binding Transcription Factor (OCT4), and the hormonal
assay was evaluated. Both groups infused with Bone Marrow Mesenchymal Stem Cells (BM-MSCs) derived exosome
and FVE have mild to moderate improvement in the histological ovarian structure by the presence of normal follicles
in different stages with normal hormonal profile and highly expressive of OCT4. BM-MSCs derived exosome and
FVE have moderate modulates the immunohistological structure of the PCOS ovaries, which may be a factor in the
maintenance of steroid-induced PCOS. |
