Background: pressure ulceration (PU) is a thought-provoking
complication of ischemia followed by reperfusion characterized by high
disability, mortality and morbidity. Vitality of wounds remains a major
challenge in forensic medicine especially in the context of suspected
neglect or abuse. Bone marrow-derived endotheliaӀ progenitor cells
(EPCs) usage has been broadly manipulated in diabetic wound patients
owing to its high contented angiogenic and growth factors.
Aim: To assess the efficacy of EPCs therapy in promoting PU healing in
relation to angiogenesis and epidermal cells regeneration based on
histological, immunohistochemical and molecular mechanisms, and to
explore its potential relevance as a supportive experimental model for
studying wound vitality in pressure-related skin injuries.
Methods: thirty adult albino rats were assigned to 3 groups [control, PU
and EPCs + PU groups]. Combines of two magnets were applied to the
skin on the backs of rats to instigate ischemia followed by reperfusion.
Histological and immunohistochemical analysis performed on the 14th
day of the experiment. The effect was evaluated by assessing dermic
collagen by Masson trichrome stain, re-epithelialization by PCNA
immunostaining and angiogenesis by CD31 immunostaining. Gene
expression of MMP, TIMP VEGF and VWF gene expression were also
evaluated. |
