Two field experiments were carried out at an organic farm (Minoufia Governorate) during 2005- 2006 and 2006-2007 seasons to study the interaction effect of biofertilization and organic manuring on dehydrogenase, polyphenol oxidase and peroxidase activity, macronutrients uptake, oil yield and oil composition of marjoram. Greater activities of dehydrogenase, peroxidase and polyphenol oxidase were obtained in soil and marjoram treated with combination of biofertilizers and organic manure than soil treated with biofertilizers or organic manure. The greatest activities of peroxidase and polyphenol oxidase were obtained with the treatment of biofertilization combined with full dose of organic manure. Similar results trend was observed with N, P and K uptake because N. P and K uptake by marjoram were greater with dual application of biofertilization and organic manure than other treatments. There is no significant difference between oil yield obtained with chemical fertilization treatment and biofertilizers combined with full dose of organic manure treatment. Fourteen compounds, accounting for more than 97 % of the total volatiles components in most marjoram samples were detected and identified. Dual application of organic manure and biofertilization to soil and marjoram produced maximum percent of terpinen-4-ol (the major compound in marjoram oil) compound in marjoram.
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