Green synthesis of silver and chitosan nanoparticles have been achieved using Nerium.oleander and Aloe
vera leaf extract as reducing agents. The formation of nanoparticles was quantified by UV-vis spectroscopy
of wave length from 200-700. TEM, XRD and FTIR were used for characterization. The present investigation
was carried out to assess the activity of antioxidant enzymes, SOD, CAT, GPx and GST in third instar larvae
of Musca domestica under the effect of methanol leaf extract, synthesized silver and chitosan nanoparticles
and the LC50 was used for biochemical assay. The activity of antioxidant enzymes in M. domestica larvae
were varied with the time where an increase in SOD activity was recorded after 24hrs in methanol extract and
AgNPs treated larvae, then decreased again after 48hrs. In CsNPs treated larvae, SOD induced a continuously
decreased activity at all times of investigation. CAT activity followed the same trend of SOD. After 48hrs of
treatment the activity of GPs was significantly decreased in all treated groups, at 1% and 5% level of
significance in larvae treated with chitosan Nerium and chitosan A. vera, respectively. After an increase in
GST activity at 24hrs, a decrease was noticed after 48hrs. From the present study, the use of N. oleander and
A. vera as methanol crude extracts to synthesize silver and chitosan nanoparticles could be a new approach
for the control of house flies. |
