| Title |
preparation and evaluation of bivalent oil emulsion inactivated IBV vaccine and its efficacy in controlling the disease in field |
| Type |
PhD |
| Supervisors |
Moustafa, Magda1, Zyan, Kamel1, ElBorai, Ibrahim1, El-Mahdy, Susan Sayed |
| Year |
2020 |
| Abstract |
Infectious bronchitis is a highly contagious disease, considered as one of the most important poultry diseases
and it causes huge economic losses in poultry industry due to its effect on broiler growth and egg production
quantity and quality in laying hens, Egyptian poultry farms suffer from frequent respiratory disease outbreaks
associated with IBV variant 2 strains. Inactivated vaccines are important component in vaccination program
of layers and breeders for egg production and also provide protection against early infection through maternal
immunity in progeny. In this study, a newly prepared inactivated classical and variant 2 vaccine was compared
for its field efficacy with commercial (ND&IB) in layer chickens. Vaccinated chickens from both groups were
evaluated against both homologous and heterologous challenge in comparison to control unvaccinated groups.
Four estimated parameters were applied in this experiment, humoral immunity after vaccination and challenge
by Enzyme linked immunosorbent assay (ELISA), egg production percentage, detection of challenge strains
by real-time RT-PCR, and histopathological findings after challenge from tracheal and oviduct samples. The
results revealed that the prepared vaccine give higher protective antibody titers in serum and better egg
production after challenge than commercial inactivated (ND&IB) one . The detection of IB viruses shedding
was limited to trachea regarded its effect on oviduct except only in control groups. The current study
demonstrates the effectiveness of locally prepared bivalent IBV vaccine in commercial layers; also
combination of inactivated variant and classical IBV strains confers broad protection against both homologous
and heterologous challenge in IBV endemic areas. |
| Keywords |
IBV, layer chickens, inactivated vaccine, variant 2 |
| University |
Benha univesity |
| Country |
Egypt |
| Full Paper |
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