Helicobacter pylori is the key pathogen for gastroduodenal diseases. The clinical outcome of H. pylori infection is influenced by the presence of strain-specific virulence factors that are usually detected by the presence of specific anti-H. pylori antibodies in serum. Apart from the detection of these antibodies by enzyme-linked immunosorbent assay (ELISA), it is desirable to obtain additional information concerning the presence of certain virulence factors of H. pylori that are detected by immunoblot analysis.
Objective :to evaluate if blotting can replace the need for invasive endoscopy , compare between it and ELISA as serodiagnostic test , and to focus on identifying factors and markers that define high-risk patients in whom H. pylori infection needs to be eradicated.
Subjects and Methods: 19 dyspepetic patients were subjected for gastroscopy to obtain antral biobsy,direct urease test and culture of biobsy on specific media . Sera were obtained from the patients for IgG examination by ELISA and western blotting. Results: Using culture and direct urease test as a gold standard to evaluate ELISA and blotting as serodiagnostic tests for H.pylori ; it was found that western blotting was more sensitive(100%) and specific(87.5%) than ELISA ( sensitivity 81.8% and specificity 75% ).Only western blotting was able to detect antibodies to virulence proteins especially cytotoxin associated antigen(CagA) and vacuolating cytotoxin antigen (VacA).Conclusion : Western blotting is a highly sensitive valuable noninvasive test to diagnose toxigenic H.Pylori infection .So that unnecessary gastroscopy and treatment can be avoided.
Helicobacter pylori is the key pathogen for gastroduodenal diseases. The clinical outcome of H. pylori infection is influenced by the presence of strain-specific virulence factors that are usually detected by the presence of specific anti-H. pylori antibodies in serum. Apart from the detection of these antibodies by enzyme-linked immunosorbent assay (ELISA), it is desirable to obtain additional information concerning the presence of certain virulence factors of H. pylori that are detected by immunoblot analysis.
Objective :to evaluate if blotting can replace the need for invasive endoscopy , compare between it and ELISA as serodiagnostic test , and to focus on identifying factors and markers that define high-risk patients in whom H. pylori infection needs to be eradicated.
Subjects and Methods: 19 dyspepetic patients were subjected for gastroscopy to obtain antral biobsy,direct urease test and culture of biobsy on specific media . Sera were obtained from the patients for IgG examination by ELISA and western blotting. Results: Using culture and direct urease test as a gold standard to evaluate ELISA and blotting as serodiagnostic tests for H.pylori ; it was found that western blotting was more sensitive(100%) and specific(87.5%) than ELISA ( sensitivity 81.8% and specificity 75% ).Only western blotting was able to detect antibodies to virulence proteins especially cytotoxin associated antigen(CagA) and vacuolating cytotoxin antigen (VacA).Conclusion : Western blotting is a highly sensitive valuable noninvasive test to diagnose toxigenic H.Pylori infection .So that unnecessary gastroscopy and treatment can be avoided.
Helicobacter pylori is the key pathogen for gastroduodenal diseases. The clinical outcome of H. pylori infection is influenced by the presence of strain-specific virulence factors that are usually detected by the presence of specific anti-H. pylori antibodies in serum. Apart from the detection of these antibodies by enzyme-linked immunosorbent assay (ELISA), it is desirable to obtain additional information concerning the presence of certain virulence factors of H. pylori that are detected by immunoblot analysis.
Objective :to evaluate if blotting can replace the need for invasive endoscopy , compare between it and ELISA as serodiagnostic test , and to focus on identifying factors and markers that define high-risk patients in whom H. pylori infection needs to be eradicated.
Subjects and Methods: 19 dyspepetic patients were subjected for gastroscopy to obtain antral biobsy,direct urease test and culture of biobsy on specific media . Sera were obtained from the patients for IgG examination by ELISA and western blotting. Results: Using culture and direct urease test as a gold standard to evaluate ELISA and blotting as serodiagnostic tests for H.pylori ; it was found that western blotting was more sensitive(100%) and specific(87.5%) than ELISA ( sensitivity 81.8% and specificity 75% ).Only western blotting was able to detect antibodies to virulence proteins especially cytotoxin associated antigen(CagA) and vacuolating cytotoxin antigen (VacA).Conclusion : Western blotting is a highly sensitive valuable noninvasive test to diagnose toxigenic H.Pylori infection .So that unnecessary gastroscopy and treatment can be avoided.
Helicobacter pylori is the key pathogen for gastroduodenal diseases. The clinical outcome of H. pylori infection is influenced by the presence of strain-specific virulence factors that are usually detected by the presence of specific anti-H. pylori antibodies in serum. Apart from the detection of these antibodies by enzyme-linked immunosorbent assay (ELISA), it is desirable to obtain additional information concerning the presence of certain virulence factors of H. pylori that are detected by immunoblot analysis.
Objective :to evaluate if blotting can replace the need for invasive endoscopy , compare between it and ELISA as serodiagnostic test , and to focus on identifying factors and markers that define high-risk patients in whom H. pylori infection needs to be eradicated.
Subjects and Methods: 19 dyspepetic patients were subjected for gastroscopy to obtain antral biobsy,direct urease test and culture of biobsy on specific media . Sera were obtained from the patients for IgG examination by ELISA and western blotting. Results: Using culture and direct urease test as a gold standard to evaluate ELISA and blotting as serodiagnostic tests for H.pylori ; it was found that western blotting was more sensitive(100%) and specific(87.5%) than ELISA ( sensitivity 81.8% and specificity 75% ).Only western blotting was able to detect antibodies to virulence proteins especially cytotoxin associated antigen(CagA) and vacuolating cytotoxin antigen (VacA).Conclusion : Western blotting is a highly sensitive valuable noninvasive test to diagnose toxigenic H.Pylori infection .So that unnecessary gastroscopy and treatment can be avoided.
Helicobacter pylori is the key pathogen for gastroduodenal diseases. The clinical outcome of H. pylori infection is influenced by the presence of strain-specific virulence factors that are usually detected by the presence of specific anti-H. pylori antibodies in serum. Apart from the detection of these antibodies by enzyme-linked immunosorbent assay (ELISA), it is desirable to obtain additional information concerning the presence of certain virulence factors of H. pylori that are detected by immunoblot analysis.
Objective :to evaluate if blotting can replace the need for invasive endoscopy , compare between it and ELISA as serodiagnostic test , and to focus on identifying factors and markers that define high-risk patients in whom H. pylori infection needs to be eradicated.
Subjects and Methods: 19 dyspepetic patients were subjected for gastroscopy to obtain antral biobsy,direct urease test and culture of biobsy on specific media . Sera were obtained from the patients for IgG examination by ELISA and western blotting. Results: Using culture and direct urease test as a gold standard to evaluate ELISA and blotting as serodiagnostic tests for H.pylori ; it was found that western blotting was more sensitive(100%) and specific(87.5%) than ELISA ( sensitivity 81.8% and specificity 75% ).Only western blotting was able to detect antibodies to virulence proteins especially cytotoxin associated antigen(CagA) and vacuolating cytotoxin antigen (VacA).Conclusion : Western blotting is a highly sensitive valuable noninvasive test to diagnose toxigenic H.Pylori infection .So that unnecessary gastroscopy and treatment can be avoided.
|
