| Abstract |
Secondary metabolites are compounds neither essential for growth nor key intermediates of the organism’s basic metabolism however playing other roles within the life of microorganisms. They’re typically found as a mix of closely connected molecules with a peculiar and rare chemical structure. Secondary metabolites extracted from living microorganisms such as antibiotics, pigments, toxins, effectors of ecological competition and symbiosis, pheromones, enzyme inhibitors, pesticides, antitumor agents and growth promoters of animals and plants, and can play great role in medicine.
The current study has been performed on three candida isolates, which were collected from Kasr Al Ainy University Hospital's Microbiology and Immunology Department. The isolate identified using MALDI-TOF system to confirm the conventional biochemical identification of the most sensitive isolate. So, by morphological, cultural, biochemical characteristics and MALDI-TOF system isolates (SAM11) was confirmed as Penicillium citrinum.
Marine microorganisms are a source of new genes, and it is probably going to prompt the revelation of new medications and targets. Secondary metabolites produced by marine microbes have yielded pharmaceutical items. So, thirteen isolates were isolated from three marine samples, thirteen fungal strains (designated as SAM11 to SAM313). Pre-screening biological anticandida activity for the ethyl acetate crude extracts of these isolates against three different candida samples (urine1- urine2- vaginal) was done by using a plate assay method. The isolated fungal acetate extracts were tested for their anticandida activity. Several isolates exhibited anticandida activity against three candida isolate but one isolate (SAM11) from the 13 fungal isolates showed potent anticandida, and were the most effective. While the most of crude isolate have a variable activity against candida isolate.
The fungal isolate (SAM11) has high anticandida activity against Candida albicans subjected to molecular identification. The identification was achieved by Morphological, physiological, biochemical and chemo-taxonomical characteristics of streptomycetes isolate H2 and Molecular identification. The sequence data obtained from the strain (SAM11) has been submitted to GeneBank. It has been confirmed that the strain is a member of the genus Penicillium spp. We obtain Penicillium citrinum strain. The strain Penicillium citrinum strain was cultivated on rice medium. After harvesting and scaling up, the obtained crude extract was purified utilizing a variety of chromatography techniques to deliver many fractions with less secondary metabolites and according to the anticandida activity of these metabolites against three candida isolates. Then, we selected a fraction that purified using a series of chromatographic techniques to obtain a pure compound with high activity against three bacterial isolates. The structures of one pure compound with high activity were verified using spectroscopic method (GC-MS).
Based on the above spectral data, bioactive compounds was identified
In our study, an interesting finding is the inhibition of the Candida albicans by one fraction of Penicillium citrinum crude extract fractionation. The results showed significant inhibition with promising anticandida parameters . The results revealed that the extract exhibited anticandida activity against the tested strains.
|
