Three different formulae of inactivated trivalent FMD vaccine serotypes (O Pan Asia1, A Iran O5 and SAT2/EGY/2012) were prepared as formula (1) prepared with Montanide ISA 206 (50% oil to 50%antigen); formula (2) prepared with carbopol (50% carbopol to 50% antigen) and formula (3) prepared with 25% Montanide ISA 206 and 25% carbopol with 50 % antigen. All of such formulae were found to be free from foreign contaminants; safe and potent showing no post vaccinal reactions and high protective levels of specific FMD antibodies. The immunogenicity of each vaccine formula was determined by vaccination of calves groups where cellular immunity was evaluated by Lymphocyte blastogenesis using XTT assay through separation of lymphocytes and determination of viable cell number in addition to estimation of interleukin IL-6 and IL-12 levels revealing higher values on the use of carbopol with Montanide oil than the use of each adjuvant alone. Also monitoring of humeral FMD antibodies in vaccinated calves revealing that obtained results and the statistical analysis of humeral antibody titers against FMDV serotypes (O; A and SAT2) induced by Montanide oils 206 with carbopol is the best vaccine formula followed by Montanide oils 206 and finally Carbopol which give early, short lasting immunity. |
