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Ass. Lect. Samaa Atef Sayed Ismail :: Publications:

Title:
Characterization of rhamnolipids produced by Pseudomonas putida ON763757 isolated from petroleum contaminated soils
Authors: Not Available
Year: 2022
Keywords: Not Available
Journal: Not Available
Volume: Not Available
Issue: Not Available
Pages: Not Available
Publisher: Not Available
Local/International: Local
Paper Link: Not Available
Full paper Not Available
Supplementary materials Not Available
Abstract:

Sixteen biosurfactant-producing bacteria were isolated from multiple petroleum-contaminated soils in Kaluybia Governorate, Egypt. Six screening tests (emulsification index (EI24%), oil spreading assay, emulsification activity, cetyltrimethylammonium bromide (CTAB), hemolytic and lipolytic activities) were conducted to select the high producer isolate. Out of 16 isolates, only one was chosen for molecular identification as the most efficient biosurfactant-producer and was found to be Pseudomonas putida ON763757. The type of biosurfactant was determine via three chromatography analytical methods, High-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FT-IR) and Gas chromatography–mass spectrometry analysis (GC-MS). HPLC analysis showed that chlorogenic acid (1409.20 µgg-1) was the main component, followed by ellagic acid (530.12 µgg-1). FTIR and GC-MS analyses indicated that the biosurfactant of Ps. putida was rhamnolipid, which is an exciting product for environmental and industrial applications.

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