This study was done to assess maternal stress of mothers having premature neonates, the study was carried out at Neonatal Intensive Care Units at Banha Hospitals. A descriptive design was utilized for this current study. The total sample was 200 mothers have premature neonates. Tow tools were used to collect the needed data; the first tool was interview sheet, consisted of demographic data about mothers, history of previous prematurity, prenatal and natal complaints, gestational age of the child, birth order of the child, and weight of the child. The second tool was parenting stress scale to measure mother's stress of their premature neonates. The study results revealed that maternal stress is highly affected by the mother's age, level of education, perinatal and natal complaints, the history of previous prematurity, as well as birth order of the child. The main psychosocial problem was financial problem, the responsibility of being a mother of premature infant, and physical appearance of infant, so several recommendation were regarding the role of health education helping the mothers to overcome their stress, effectively way to cope with stress and how to deal with their premature infants
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