Opuntia ficus-indica peels contain a number of active substances. Bioactive secondary metabolites have effective role in the maintenance of health and the treatment of some common disorders. The chemical composition showed moisture (6.78±0.065), total protein (4.78±0.030), fats (0.9±0.010), total ash (10.49±0.233), total carbohydrates (50.12±0.045), and crude fiber (26.93±0.025) on the dry weight. Total phenolics in the methanolic and ethanolic extracts of Opuntia ficus indica peels were (292.9±0.0198) and (165.6±0.0553) mg GAE/100 g, while total flavonoids were (20.39 ±0.0031) and (11.39±0.0346) mg QE/100g, respectively. Antioxidant activity in the methanolic extract was (79.100±0.005), while in the ethanolic extract it was (35.09±0.003). Using HPLC analysis, identification of thirteen phenolic compounds were: Chlorogenic acid, Catechin, Methyl gallate, Coffeic acid, Pyro catechol, Coumaric acid, Ferulic acid, Naringenin, Daidzein and Querectin. While the highest concenterations were Pyro catechol and Catechin which have concentrations 60.36 µg/ml and 58.47µg/ml respectively. LC/MS/MS analysis identified the main phenolic compounds (5peaks) in opuntia peel ethanolic extract as piscidic, eucomic, malic, iso-rhamnetin and behenic acid.
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