BMP7 is a member of transforming growth factor-β (TGF- β) superfamily which plays an important role in the growth and follicular development in mammalian ovary. The present study was designed to amplify and detect polymorphism in BMP7 (exon 6) and BMP 7 (Part of intron 6 plus part of exon7) genes associated with infertility in Egyptian buffalo cows. PCR amplification of DNA isolated from blood of 35 Egyptian water buffalo cows (5 normal fertile, 16 repeat breeders and 14 anestrum) reveals the expected size (259 bp) for exon 6 and (360 bp) for (Part of intron 6 plus part of exon7). Polymorphisms in these two loci were studied by using direct sequencing andRFLP technique.No SNP was detected in exon 6, a monomorphic restriction banding pattern observed in normal cyclic and infertile animals. Non- synonomous SNP (G/A) at base 82 from the start of exon 7 was discovered in BMP7 gene. G to A transition revealed two genotypes GG and GA in the investigated Egyptian water buffaloes.The GG genotype was predominant with a genotype frequency of 0.94. GA genotype was found in some infertile animal with frequency of 0.06. Thus,BMP7 gene (exon 7) polymorphism can select efficiently in marker-assisted selectionin breeding programs for the Egyptian water buffalo. |
