In the present study, the effects of thyme supplementation on lipid peroxidation and antioxidant enzymes in chronic intoxicated rats with organophosphorous pesticides have been evaluated. This study was carried out on 60 male rats. The rats were divided into four equal groups. 1) Normal group (N): received no drugs. 2) POM group (P): received single oral dose of POM daily for 3 months. 3) Thyme group (Thy): received single dose of Thyme per os daily for 3 months. 4) Thyme + POM group (Thy + P): received single dose of POM + Thyme per os daily for 3 months. Blood and brain samples were collected from all animal groups three times at one, two and three months from the onset of experiment and used for determination of antioxidant enzyme activities, non enzymatic antioxidant (GSH and Ceruloplasmin) and free radicals (MDA and NO). Moreover, AChE activity in erythrocytes and brain were also determined. Pirimiphos methyl induced a significant decrease in antioxidant enzyme activities and erythrocytes and brain AChE. Also marked depletion in erythrocytes and brain GSH, were observed. Moreover, administration of Pirimiphos methyl exhibited a significant increase in serum ceruloplasmin and erythrocytes SOD and serum GGT activities in addition to a marked increase in erythrocytes and brain MDA and serum NO levels. While administration of thyme to rats received oral dose of POM exhibited a significant increase in antioxidant activities, GSH, ceruloplasmin and a marked decrease in erythrocytes and brain MDA and serum NO. These results concluded that, thyme constitutes a powerful antioxidant property that was able to improve the adverse biochemical changes induced by organophosphorous compounds that are widely in our country.
Key Words: Antioxidants, brain, erythrocytes, organophosphorous pesticides, oxidative stress, Pirimiphos methyl, Thyme.
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