For the present study the harmful effects of organophosphorous pesticides chronic toxicity on several biochemical parameters and evaluation of the possible protective effects of chlorophyllin as antioxidant in rats exposed to Pirimiphos methyl (POM), one of organophosphorous pesticides widely used in the middle east, were investigated. Sixty adult white male albino rats weighting 150 - 200 gm were used in this study. The rats were divided into four equal groups. 1) Normal group (N): received no drugs. 2) POM group (P): received single dose of POM daily for 3 months. 3) Chlorophyllin group (Chl): received single oral dose of Chlorophyllin daily for 3 months. 4) Chlorophyllin + POM group (Chl + P): received single dose of POM + Chlorophyllin per os daily for 3 months. Blood and brain samples were collected from all animal groups three times at one, two and three months from the onset of experiment. Plasma was separated for determination of Ceruloplasmin, NO and γGT. Moreover, after plasma separation, erythrocytes were washed for determination of AChE, GSH, MDA, CAT, SOD, GPx, GR and GST. Brain AChE, GSH, MDA, CAT, were also determined. The obtained results revealed that, administration of Pirimiphos methyl exhibited a significant decrease in erythrocytes and brain AChE, CAT, GSH, erythrocytes GPx, GR and GST activitie,. CHL administration in POM intoxicated rats exhibited significant increase in all mentioned parameters. On the other hand, a marked increase in erythrocytes SOD, serum GGT activities, erythrocytes and brain MDA and serum NO level were observed. CHL administration in POM intoxicated rats exhibited significant decrease in all mentioned parameters. From the obtained results it could be concluded that, the potential of CHL as natural antioxidant act as a powerful agent against the toxic effects of POM.
Key Words: Antioxidants, brain, Chlorophyllin, erythrocytes, organophosphorous pesticides, oxidative stress, Pirimiphos methyl.
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