A total number of 28 mature Barki ewes were divided into three groups according to the methods used for synchronization of estrous cycle with different drugs. Group I comprised 8 ewes received no drugs and served as control group. Group II included 10 ewes synchronized by 3 ml (15 mg) prostaglandin F2 injected i.m and repeated once after 9 days from the first injection.Group III comprised 10 ewes synchronized by application of intra vaginal sponge impregnated by 60 mg medroxy progesterone acetate for 13 days. Blood samples were taken from all groups during estrus and diestrus phases, twice a week during natural, synchronizing estrus and at the next one for ewes were not conceive. The results showed that, the percentage of conception rate were 75%, 30% and 87.5% in first estrus and 100%, 70% and 100% in the second one for group I, II and III, respectively. Ewes synchronized by PGF2 (group II) showed a significant decrease in serum globulins and total lipids concentrations whereas the value of serum copper level and serum ALT activity showed a significant increase during the follicular phase in comparison with control group. Meanwhile,during the luteal phase, serum glucose, total lipids, copper and progesterone concentrations showed a significant decrease. But serum total cholesterol level, ALT activity, calcium and potassium concentrations were significantly higher when compared with the control group (group I). In ewes synchronized by Veramix vaginal sponge (group III) the obtained results showed that, during follicular phase serum total protein and total globulin levels were significantly decreased but the concentrations of serum total lipids, total cholesterol, calcium and ALT activity showed a significant increase. Moreover, during luteal phase serum glucose and copper levels, AST and ALT activities showed a significant decrease in comparison with control group. However, Ewes synchronized by PGF2 showed low fertility at first estrus than second one. Also some blood parameters as glucose, total protein, total globulins, total lipids, calcium, sodium and progesterone concentrations showed a significant decrease, while serum AST and ALT activities showed a significant increase at first estrus than second one. The results indicated that, the use of vaginal sponge impregnated with medroxy progesterone acetate as an estrus synchronizing agent in ewes was effective and beneficial than the PGF2 injection in respect to conception rate. |
