A total number of 60 anestrous balady cows, divided into two main groups according to the ovarian findings (30 with persistent CL and 30 with smooth inactive ovaries)., Each main group was divided into 3 subgroups according to the hormonal treatment used. The first one, injected with 25 mg PGF2 (Lutalyse) two doses 11 days a part. The second subgroup injected with 0.25 mg PMSG (Folligon) and the third injected with 25 mg PGF2 (Lutalyse) plus 0.25 mg PMSG (Folligon). Estimation of serum progesterone, concentration, estradiol 17-B, thyroid hormones (T3 and T4) and some serum biochemical constituents as inorganic phosphorus, total cholesterol, glucose and total protein concentrations were performed for all animals before and at the appearance of heat signs. The obtained results revealed that, balady cows with persistent CL showed a highly significant decrease in serum progesterone and glucose level after all hormonal treatment. However, a highly significant increase in estradiol -17, T3, T4, inorganic phosphorus and total cholesterol concentrations were observed after treatments with PGF2, and/or PMSG. On the other hand, there was a significant increase in serum total protein level after treatment with PGF2, PMSG alone and PGF2 plus PMSG. Concerning the cow with smooth inactive ovaries, there were highly significant increase in level of serum progesterone and Estradiol -17 after all regimes used. There were highly significant increase in levels of serum T3, T4, were noticed. Whereas, serum total cholesterol and glucose were highly significantly decreased after all regimes. The values of serum inorganic phosphorus showed a highly significant increase after treatment with PGF2 alone and PGF2 plus PMSG. Moreover non significant increase in values of serum inorganic phosphorus observed after treatment with PMSG alone. The mean value of serum total protein level showed a significant increase after treatment with PGF2 alone and PMSG alone. Besides, there was a highly significant increase in the level of total protein after treatment with PGF2 plus PMSG. The present study revealed also that, higher percent of conception rate (70% and 60%) after 1.6±0.22 and 1.9±0.23 number of services recorded in animals treated with PGF2 plus PMSG for both main groups having persistent CL and smooth inactive ovaries, respectively. It could be concluded that injection of PGF2 followed by PMSG 5 days later might give higher conception rate and lower number of services per conception in balady cows with persistent CL and smooth inactive ovaries. |
