In the present study, the potential protective and therapeutic effect of rutin (RUT) administration on serum nitric oxide (NO),tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), colon tissue lipid peroxidation, antioxidant enzymes,reduced glutathione (GSH),and myeloperoxidase (MPO) in trinitrobenzenesulfonic acid(TNBS)-induced ulcerative colitis in ratshave been evaluated. Fourty male albino rats were divided into four equal groups of 10 rats each. Group Ι :( Control group): received no drugs. GroupΠ:( ulcerative colitis -induced group): Administered single intra-colonially dose of 150 mg/kg of TNBS for ulcerative colitis induction. Group III:( ulcerative colitis+ RUT protected group):received RUT (200 mg/kg body weight/day) orally for 21 days prior TNBS administration for ulcerative colitis induction.Group IV :( ulcerative colitis + RUT treated group): treated with RUT as in group III for 21 days after ulcerative colitis induction. Blood samples and colon tissue were collected at the 22th day from the onset of RUT administration. The obtained results showed that,TNBS-inducedulcerative colitiscaused significant decreased in serumNO level andGlutathione peroxidase (GPx), Superoxide dismutase (SOD), Glutathione –S- transferase (GST) andcatalase (CAT) activities in colon tissue. On the other hand,a marked increasein colon tissueGlutathione reductase (GR) and MPO activities and GSH andL-Malondialdehyde (L-MAD) concentrations and in serum TNF-α and IL-1β levelswere observed in TNBS induced colitis in rats.
Rutin was able to mitigate colon mucosa damage induced by TNBS through increasing of NO, GPX, SOD, CAT, and GST in addition todecreasing L-MDA, TNF-α, IL-1β and MPO activity in colon tissue. Theseresults suggest that,rutin may be effective in enhances the healing of ulcerative colitis by its radical scavenging and anti-inflammatory effect, inhibited neutrophil accumulation, and regenerating endogenous antioxidant mechanisms.
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