The present study was undertaken to evaluate the potential protective and therapeutic effect of rutin (RTN) administration on ethanol-induced gastritis in rats. Fourty male albino rats were divided into four groups. Group Ι :( Control group): received no drugs. Group Π :( Gastritis non-treated group): administered with a single oral dose of 1 ml/rat of absolute ethanol for gastritis induction. Group III :( Gastritis + RTN protected group): received RTN (200 mg/kg body weight/day) orally for 14 days prior ethanol administration. Group IV :(Gastritis + RTN treated group) received RTN as in group III and the treatment was continued for 7 days later.
Blood samples for serum separation and gastric tissue were collected at the 15th. and 22th. days from the onset of RTN administration for determination of serum nitric oxide (NO) and sialic acid (SA), gastric tissue Glutathione peroxidase (GPx), Superoxide dismutase (SOD), Catalase (CAT),Glutathione reductase (GR), reduced glutathione (GSH), L- Malondialdehyde (L-MDA), vitamin C, DNA-fragmentation and myeloperoxidase (MPO). The obtained results showed that, ethanol induced gastritis caused a significant decrease in serum NO, SA, and gastric tissue GSH, vitamin C concentrations and GPX, SOD, GR and CAT activities. However, MPO activity and DNA-fragmentation were significantly increased. Administration of RTN was able to mitigate gastritis induced by ethanol through increasing of NO, SA, GSH, vitamin C concentrations, GPx, SOD, GR, CAT activities in addition to decreasing DNA-fragmentation and MPO in gastric tissue. These results suggest that, RTN may be successful in the therapeutic of gastritis by its radical scavenging and antiapoptotic activity, inhibited neutrophil accumulation and regenerating endogenous antioxidant mechanisms.
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