The objective of this study is to investigate the neuroprotective and antioxidant effects of subsequent pretreatment with curcumin as natural antioxidanton biomarkers of oxidative stress and inflammatory response brain tissue induced by endotoxemia.This study was carried on 42 male rats . The ratswere divided into three groups.Group 1(control) 12 ratsnot receive drugs, Group 11 15 rats injected intraperotineally (i.p) with a single dose of Lipopolysaccharide (LPS) at a dose 200mg/kg b.wt, Group III 15 ratsadministered Curcumin orally at dose 100 mg/kg/b.w/day for 3 weeks and then injected with a single dose of LPS at dose 200 mg/kg/b.w. Blood samples for serum separation and brain tissue were collected from all animal groups twiceat one and three hours from the onest of injection with Endotoxin. All sera were subjected directly for determination of Tumor Necrosis Factor (TNF), Interleukin -6 (IL-6), Sialic acid (SA) and Nitric oxide (NO).In addition determination of brain tissues of Glutathione peroxidase (GPx)Superoxide Dismutase (SOD), Catalase (CAT) and L-MDA.The obtained result revealed that ,Endotoxemia could be potientially significant increase serum TNF, Il-6and brain L-MDA.Alsoinduced a significant decreased in SA, NOand GPX, CAT and SOD in brain tissues.Treatment with curcumin significantlydecreased in serum TNF, IL-6, and L-MDA in brain tissue .Also induced significantly increased in serumNO,SA and GPX, CAT and SODin brain tissue.From the obtained results it could be cocluded that curcumin protects against lipid peroxidation,oxidative stress and decrease the inflammatory response to endotoxin injection. |
