The high-fructose diet induces insulin resistance, hyperinsulinaemia, hyperglycemia, alterations in lipid metabolism, and oxidative stress in rat tissues which Oxidative stress plays a vital role in pathology associated with insulin resistance. The potential protective effect of resveratrol (RSV) administration on some metabolic alterations, inflammation and oxidative/ antioxidant status in rats fed with high fructose diet were evaluated. Insulin resistance was induced by feeding high fructose diet (60 g/100 g). Forty-five male albino rats divided into three equal groups. Group Ι: (Control group) rats received control normal diets. Group II (fructose-fed group) rats received fructose- enriched diet (60 g /100g). Group III: (fructose + RSV group) rats received high fructose diet and administered RSV (0.5 mg/Kg body weight /day, intraperitoneally). Blood samples and liver tissue were taken and processed for the biochemical determination. Additionally, liver and pancreatic tissue specimens were excised for histopathological examination. The obtained results revealed that, a significant increase in serum glucose, insulin, insulin resistance, total cholesterol, triacylglycerols, TNF-α and IL-6 concentrations and liver tissues L-MDA and NF-кB with marked decrease in serum adiponectin level and liver tissues CAT activity and GSH concentration were observed in high fructose diet induce insulin resistance in rats. However, resveratrol treatment to high fructose fed rats reduced the effects of high fructose and significantly improves of all serum parameters level and was able to attenuate dyslipidemia, inflammation and insulin resistance, mitigated the oxidative stress and enhanced antioxidant status in liver tissues. Interestingly, the histopathological results supported that resveratrol markedly reduced the deleterious effect induced by high fructose diet and preserved the normal histological architecture of the liver and pancreatic tissues. These results suggest that, resveratrol is effective in improving the high fructose induced oxidative stress, inflammation and insulin resistance in male rats. Also, administration of resveratrol to rats fed a high fructose diet prevents the development of oxidative stress and its associated complications include hyperglycemia, hyperinsulinemia and dyslipidemia. |
