The objective of the present study was to investigate the bioactive constituent of diethyl ether
extract and to determine whether the diethyl ether extract of Lotus peregrinus L. exerts chemoprotected
effect by stimulating apoptosis during medroxyprogesterone acetate (MPA)-DMBA
mammary carcinogenesis using the expression of the apoptosis-associated proteins Bax and
caspases 9; as well as improvement of oxidative status of the mammary tumor tissue. Sixty female
wistar rats (52 days old) were divided into four equal groups. Group I (normal group): rats
administered distilled water. Group II (carcinogenic group); on day 0, wistar rats were given DMBA
(2.5 mg/ rat; orally) followed by second dose DMBA (2.5 mg/ rat; orally) on day 15, 90-day timedrelease
pellets containing 25 mg MPA were implanted’ into rats on day 30. Group III (diethyl ether
protected), on day 0, wistar rats were given DMBA (2.5 mg/ rat; orally) followed by second dose
DMBA (2.5 mg/ rat; orally) on day 15, on day 26 rats were administered diethyl ether (50 mg /kg.
b. wt.; orally), 90-day timed-release pellets containing 25 mg MPA were implanted into rats on day
30. Group IV (diethyl ether treated group); given diethyl ether (50 mg /kg. b. wt.; orally) after
induction of mammary cancer. Our findings showed the positive treatment of mammary tumor and
improved oxidative status of the tissue was evident by increase in the levels of GSH, Catalase and
decreased levels of serum MDA levels which in turn is an indicator of reduced oxidative stress of
the tissue. Therefore, this study suggests that diethyl ether extract of Lotus peregrinus can be useful
for the treatment as well as improvement of oxidative status of the mammary tumor tissue .Also,
was more effective in inhibiting DMBA-MPA induced mammary tumors and modulating the
expression of apoptosis associated proteins. |
