The present research aimed to evaluate the hepato/neuroprotective effects of Rutin and Resveratrol as
natural antioxidants on brain and liver tissues of experimental rats exposed to acute liver failure
induced by i.p. administration of Thioacetamide (TAA), Through evaluation of plasma and brain
Ammonia, serum Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), Alkaline
Phosphatase (ALP) and Gamma Glutamyl-Transferase (γ-GT), Albumin, Total Protein, Total
Bilirubin, Urea and Uric acid. Levels of reduced glutathione (GSH) and activities of Superoxide
Dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GPx), were determined in the liver and
brain tissues. Extent of oxidative stress was also assessed by hepatic and brain lipid peroxides (MDA),
in addition to brain nitric oxide (NO) and Monoamine oxidase (MAO). Thioacetamide induced a
significant increase in 1) ALT, AST, ALP, γ-GT, Albumin; Total Protein, Total Bilirubin, Urea and
Uric acid Levels in serum, 2) plasma and brain ammonia level 3) brain NO level, 4) liver and brain
MDA. Also marked depletion in liver and brain GSH, CAT, SOD, GPx and brain MAO were
observed after TAA intoxication. Rutin and Resveratrol Pretreatment was able to mitigate hepatic and
brain damage induced by TAA and showed pronounced curative effect against lipid peroxidation and
deviated serum enzymatic variables as well as maintained glutathione status and antioxidant enzymes
toward control levels. Pretreatment of rutin and resveratrol was highly effective and protective against
TAA induced hepatic encephalopathy. The results of the present study suggest that rutin and
resveratrol have potential to exert curative effects against liver injury. |
