Background: MiRNAs are critical in the course and prognosis of the disease
and may aid in developing innovative therapies. Due to miRNAs instability and
complicated environment, which includes in vivo degradation by nucleases,
deregulation of miRNA expression is the hallmark of liver cancer. MiRNAs
can function as oncogenes or tumor suppressors; miRNA-222 promotes tumor
progression by cancer-related biological processes. MiRNAs are a novel
method for cancer gene therapy due to their influence on cell signaling
pathways by inhibiting or promoting many related genes. The interaction
between genetics and cellular pathways contributes significantly to the onset
and progression of HCC
This study aims to illustrate molecular insights into the biological basis of
potential anticancer efficacy of anti-microRNA in HCC cell lines
Results: Normalization of dysregulated miRNAs by down-regulation block
HCC cell proliferation or increase the sensitivity of liver cancer cells to
chemotherapy. Effect of UTMD-mediated with anti-microRNA-222 leads to
cell proliferation inhibition and cell apoptosis induction in HepG2 cells line.
Inhibition of miR222 expression leads to cell cycle arrest and activation of its
target genes.
Conclusion: Ultrasound microbubbles are frequently utilized to investigate
gene and miRNA functions. Gene delivery of miR-222 inhibitor by
microbubbles/ultrasound is a new therapy and overcome chemotherapy side
effects. |
