Background. Many gene polymorphism of inflammatory
cytokines have been studied and shown to
be involved in acne vulgaris. Due to ethnic variations
and gene environment interactions, gene
polymorphisms studies should be conducted in
different races.
Aim of the work. We aimed to investigate the association
between IL-8- 251T>A gene polymorphism
and the susceptibility and severity of acne vulgaris.
Subjects and methods. 100 acne vulgaris patients
and 100 apparently healthy matching control subjects
were recruited from the outpatient clinic of
Dermatology Department of Benha University.
Written informed consent was obtained from all
participants. Patients were classified into mild,
moderate and severe acne according to Global
Acne Grading system. Blood sample was collected
and DNA was extracted from the peripheral
blood using Quick-gDNATM MiniPrep kit.
Genotypes of IL-8- 251T>A were analyzed by
restriction fragment length polymorphism RFLPPCR
method.
Results. The study included 100 AV patients and
100 healthy matching controls. Thirty five percent
of patients had mild, 39% had moderate and 26%
had severe acne. Sun exposure was the most
prevalent precipitating factor in our patients. Post acne scars were detected in 31% of the patients.
The observed genotype frequencies among the
controls and cases were in concurrence with
Hardy-Weinberg equilibrium Taking AA genotype
as a reference for IL8 SNP, AT, TT genotypes and T
allele were associated with risk of acne development.
No significant association was found
between IL8 genotypes and the studied parameters
in all studied cases.
Conclusion. IL-8- 251T>A gene polymorphism may
be associated with acne development. |
