Women after menopause have a lot of complains that negatively distress their life. In the modern years, a lot of
conductions methods have been presented to relief of undesirable symptoms. Bone marrow mesenchymal stem
cells (BM-MSCs) can be recently used as a new therapeutic method to treat and avoid the hormonal therapy
symptoms and complications that used after menopause.
Aim: The aim of this research is to identify a new approach in modification of structure of vaginal mucosal
atrophy by uses of (BM-MSCs) in induced ovariectomized rats.
Methods and Results: Fifty female albino rats were used and divided randomly into five groups: control group,
ovariectomized group, ovariectomized group plus estrogen (20 ug/kg/day for 4 weeks), ovariectomized with BMMSCs
(107 MSCs/rat intravenously) and ovariectomized rats with stem cells/BM-MSCs Intra-vaginal group, the
expression of genes for GAPDH, iNOS and TGF-β were done and vaginal biopsies were taken for histological and
immunohistochemical studies. In ovariectomized group there was inflammation, ulceration and delayed re
epithelialization with irregularity in collagen fibers, decrease estrogen receptor expression and expression of TGF-β,
GAPDH and iNOS were very high, while the rate of healing of epithelium with increase, the vasculatures of vaginal
mucosa, the estrogen receptors expression were high and decrease expression of GAPDH, iNOS and TGF-β in
ovariectomized rats with stem cells/BM-MSCs Intra-vaginal group.
Conclusions: Using the BM-MSCs could be used intravaginal safely in case of vaginal atrophy as they modify
the structure of vaginal mucosa than using estrogen hormone for long time. |
