The mechanisms used to detect the role of Spn-5 gene in development of Drosophila melanogaster were selecting some genetic mutations which related to pathway serine protease inhibitors. The FLP / FRT system induction site specific mitotic recombination was used to generate recessive homozygous while the rest of Drosophila body remains heterozygous. Also, Using Ey-FLP technique helped us to identify Spn-5 gene function in eye development. The GAL4/UAS system can be used to screen for miss or overexpression of our gene of interest phenotype.
The stock of the (Udo Hacker lab) carrying lethal Piggy-Bac insertion recombined to FRT located on the third chromosome of Drosophila melanogaster was screened in this system. We found in the screen necrotic tissue melanization and tumors in different organs i.e. head, eye, wing, abdomen and tergum. The induced tumor also varied in size and shape as well as the nature of origin.
This study confirmed the wild ability to use Drosophila as a standard model system in genetic studies. The gene was identified using FLP/FRT technique. Clones of Spn-5 mutant cells are associated with wing margin defects, suggesting that Spn-5 is required for wg signaling.
Moreover, the gene (Serpin-5); is known to play many roles during eye development, in cell proliferation and could be required for photoreceptor cell determination patterning, as well as in ommatidial differentiation. the gene was found in the screen have a high potential to cause tumor.
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