Objective: Comparing different effects of supplementation of Ham’s F10 media with
vitamin E, Baker’s Yeast (Saccharomyces cerevisiae) extraction and different
concentrations of vitamin C on sperm parameters.
Patients and methods: 40 ejaculated semen samples of infertile men were obtained.
Each semen sample was divided into 6 equal fractions. A Control group (activated
with Ham’s F10 only). 5 study groups were activated with Ham’s F10 plus: Group 1:
Vit E (conc. 2 mg/ml Ham’s F10), groups 2,3,4 (Vit C1, Vit C2 and Vit C3) (conc.
0.02, 0.04, 0.06 mg/ml Ham’s F10, respectively) ), Group 6: Yeast ( 20 mg/ml Ham’s
F10).
Results: Sperm total motility, progressive motility, vitality and oxidative stress were
significantly increased in treated groups than the control group. The results showed
that Vit. E has the best results (72%, 45%, 95%& 0) with comparable results with
Yeast group (66%, 39%, 95%& 0) and the best result for Vit C was with conc. (0.06
mg/ml) in group Vit C3(65%,39%, 95%& 0), respectively.
Conclusions: Vit. C, E and yeast, all have beneficial effects on semen parameters. Vit.
E has the best results than Vit. C and yeast. The best concentration of Vit. C to achieve
results is 0.06 mg/ml Ham’s F10.
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