The current investigation was carried out at Nubaria Agricultural Research Station, Agricultural Research Center, Egypt, during 2015/16 and 2016/17 seasons to evaluate 20 faba bean (Vicia faba L.) promising lines and five cultivars ( Misr 3, Nubaria 1, Giza 843, Sakha 3 and Giza 716) under soil salinity stress. The entries were sown in a randomized complete block design (RCBD) with five replicates; the EC (dS m-1) of the soil was 6.67 in the first season and 4.35 in the second one. The promising line M6 produced the greatest numbers of branches (4.13 and 4.80), pods (10.47 and 36.80), seeds (29.20 and 102.00) and seed yield plant-1 (18.58 and 70.82 g) in the two seasons, respectively. Meanwhile, , Giza 843 cv. Recorded almost similar pod number (10.60 and 22.20), seed number (24.5 and 70.8) and seed yield plant-1(17.56 and 64.94 g.), in the two seasons, respectively. Therefore, promising line M6 should be further evaluated in the advanced yield trials to release it as a new cultivar, to be grown under soil salinity stress. The correlation coefficient confirmed the highly significant positive correlation (0.98**) between both of No. of pods plant-1 and seed yield plant-1 with No. of seeds plant-1. The estimated value of heritability in broad sense was high for all characteristics in both seasons except for No. of branches plant-1 in the first season, which was low (30.0). High heritability indicated that selection based on means would be successful in improving these traits. Expected genetic gains from selecting the top 5% of the characteristics under study as a percent of the mean was high except for plant height in the second season (18.5 cm) and No. of branches plant-1 in the first season (9.2). High heritability estimates along with high genetic advance for seed yield and its attributes indicated an additive gene action in its inheritance. Total protein fractionation using SDS-PAGE was used. SDS-PAGE analysis of the investigated faba bean protein has demonstrated a great heterogeneity in the separated protein fractions. Proteins have specific bands referred to different saline conditions and differences within genotypes. Three RAPD primers were successful in generating reproducible and reliable amplicons for the twenty five faba bean genotypes. |
