Abstract: Problem statement: Similar to obese patients and type 2 diabetic patients, adiponectin
levels are reduced in former Gestational Diabetes Mellitus (GDM) patients and are lower in GDM
women during late pregnancy compared with pregnant control subjects matched for BMI. Diabetic
insult at later stages in gestation, such as may occur in gestational diabetes, will foremost lead to shortterm
changes in a variety of molecules for key functions including gene expression in the placenta.
Approach: In this study we assessed the expression of adiponectin receptors in human placenta to
identify the site (s) of expression and to clarify the effect of gestational diabetes in this expression.
This study was carried on 10 normoglycemic pregnant women and 20 GDM women. The placental
tissue was collected immediately after delivery and tissue biopsies were taken from both fetal and
maternal sides of each placenta. One step-RT-PCR for ADIPOR1 and ADIPOR2 was done by Real
Time PCR using Syber Green technique. Relative quantification of mRNA of the ADIPOR1 and
ADIPOR2 genes was measured using ABI7900 Real Time machine. Results: Both types of
Adiponectin Receptors (ADIPOR1 and ADIPOR2) are expressed in human placenta. ADIPOR1 is
more highly expressed than ADIPOR2 in both fetal and maternal sides of GDM cases and normal
pregnant women. ADIPOR1 mRNA expression was significantly up regulated in GDM women
compared to normal pregnant women, whereas no significant difference in the expression of ADIPOR2
was detected between the two groups. There was no evidence of maternal-fetal side difference in the
expression of adiponectin receptors in GDM cases but in normal pregnant women there is a
statistically significant difference between both sides in the expression of both ADIPOR1 and
ADIPOR2. Conclusion: We concluded that adiponectin plays an important role in mediation the |
