bleomycin-induced lung toxicity and oxidative damage by decreasing the deactivating enzyme, genetic
vulnerability, and released cytokines of inflammation. Melatonin has a free radical detoxifying effect, coenzyme Q10
has a strong antioxidant effect. Aim of the study: this study aimed to evaluate the possible protective role of melatonin
and coenzyme Q10 in bleomycin-induced lung injury in Albino rats. Material and Methods: forty male Albino rats
were categorized into five groups; group I (control group), group II (bleomycin group): rats were given a single dose
of bleomycin intra-tracheal for inducing lung injury, group III (melatonin group): rats were given melatonin for three
weeks after intratracheal installation of bleomycin, group IV (coenzymeQ10 group): rats were given coenzymeQ10
for three weeks after intratracheal installation of bleomycin and group V (combined melatonin and Co Q10 group):
rats were given a combination of melatonin and coenzyme Q10for three weeks after induction of bleomycin lung
toxicity. Lung tissues were prepared for biochemical, histological and immunohistochemical studies.
Results: bleomycin produced a significant increase in the level of malondialdehyde and a significant reduction in
glutathione peroxidase activity in lung tissues with loss of normal histological lung architecture, significant elevation
in main area percent of collagen fibers deposition and caspase-3 immuno positive expression. In group III melatonin
enhanced a significant improvement in the biochemical changes, moderate prevention of histopathological changes in
lung tissue with a significant reduction in main area percent of collagen fibers deposition and caspase-3 immuno
positive expression. While, in group IV co enzyme Q10 enhanced non significant improvement in the biochemical
changes, mild prevention of histopathological changes and non-significant reduction in main area percent of collagen
fibers deposition and caspase-3 immuno positive expression. Using a combination of both drugs in group V enhanced
a significant improvement in the biochemical changes and almost preservation of normal histological architecture of
the lung tissue. Conclusion: administration of both melatonin and coenzyme Q10 produced almost a complete
recovery of bleomycin induced lung injury |
