Platelet rich plasma (PRP) and platelet rich fibrin (PRF) are considered excellent
concentrated sources of growth factors (PDGF, TGF-β, VEGF, bFGF) and cytokines
fundamental for wound healing. The aim of this study was to evaluate and compare the effect
of PRP and PRF on the second intention wound healing through clinical vision,
histopathological examination, and changes in interleukin 10 (IL10) and transforming growth
factor-β (TGF-β) genes expression. Fifteen adult dogs were used in this study: induction of a
3 cm diameter total thickness cutaneous injury at the right chest region. The animals were
divided equally into three groups, and the wounds were treated twice weekly for three
successive weeks. The first group (Group A) received only normal saline (control group).
The second group was treated by PRP (group B). The third group received PRF treatment
(group C). Clinical evaluation, molecular studies of IL10 and TGF-β gene expression, and
histopathological examination were used to demonstrate the difference between the three
treatment regimens. Results showed a non-significant negative correlation between weight
loss and wound healing rate (WHR%), but a significantly high positive correlation between
treatment cost either by PRP or PRF with IL10 (0.79*) and WHR% (wound healing rate)
(0.994**). The IL10 significantly increased in PRP group, while TGF-b significantly
increased in the PRF group. This study concluded that the PRP and PRF exhibited higher
regeneration capacity and accelerated the quality of wound healing. |
