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Dr. Zeinab Reda Ahmed Abo Ezz :: Publications: |
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| Title: | A Recent Non cytopathogenic bovine viral diarrhea virus detected by immunofluorescence and reverse transcriptase polymerase chain reaction |
| Authors: | Samia Abdelkareem, Ayman Said El-Habbaa, Zeinab RA Aboezz, Ehab ElNahas |
| Year: | 2021 |
| Keywords: | Not Available |
| Journal: | Benha Veterinary Medical Journal |
| Volume: | 41 |
| Issue: | 1 |
| Pages: | 39-41 |
| Publisher: | Benha University; Faculty of Veterinary Medicine |
| Local/International: | Local |
| Paper Link: | |
| Full paper | Not Available |
| Supplementary materials | Not Available |
| Abstract: |
In the present study, a non-cytopathogenic bovine viral diarrhea virus (BVDV) field strains from bovine semen samples were detected by immunofluorescence (IF) and reverse transcriptase polymerase chain reaction (RT-PCR). Two out of five semen samples tested positive for the presence of BVDV antigen employing antigen capture ELISA kits. The two semen samples were injected in Madin-Darby bovine kidney (MDBK) cells in a trial for BVDV isolation. The chosen two samples were not cytopathic and subjected for further identification and genotyping using one step reverse transcriptase polymerase chain reaction (RT-PCR) assay. The isolated BVDV field strains from semen were found to be BVDV genotype 1. |
