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Prof. Dr.Mahran Mokhtar Mohamed Ashry El Nagar :: Publications:

Transcriptome analysis and codominant markers development in caper, a drought tolerant orphan crop with medicinal value
Authors: 1. Mercati, F., Fontana, I., Gristin, A., Martorana, A., El Nagar, M., De Michele, R., Fici, S. and Carimi, F.
Year: 2019
Keywords: Not Available
Journal: Scientific Reports
Volume: 9
Issue: 10411
Pages: 1-16
Publisher: Not Available
Local/International: International
Paper Link:
Full paper Not Available
Supplementary materials Not Available

Caper (Capparis spinosa L.) is a xerophytic shrub cultivated for its flower buds and fruits, used as food and for their medicinal properties. Breeding programs and even proper taxonomic classification of the genus Capparis has been hampered so far by the lack of reliable genetic information and molecular markers. Here, we present the first genomic resource for C. spinosa, generated by transcriptomic approach and de novo assembly. The sequencing effort produced nearly 80 million clean reads assembled into 124,723 unitranscripts. Careful annotation and comparison with public databases revealed homologs to genes with a key role in important metabolic pathways linked to abiotic stress tolerance and bio-compounds production, such purine, thiamine and phenylpropanoid biosynthesis, α-linolenic acid and lipid metabolism. Additionally, a panel of genes involved in stomatal development/ distribution and encoding for Stress Associated Proteins (SAPs) was also identified. We also used the transcriptomic data to uncover novel molecular markers for caper. Out of 50 SSRs tested, 14 proved polymorphic and represent the first set of SSR markers for the genus Capparis. This transcriptome will be an important contribution to future studies and breeding programs for this orphan crop, aiding to the development of improved varieties to sustain agriculture in arid conditions.

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