||The current study aimed to investigate the values of some blood indices and hormonal levels in the prognosis and/or diagnosis of some reproductive problems (pyometra and dystocia) in bitches and queens under Egyptian conditions.
Chapter Ⅱ aimed to address the relation between pyometra, and oxidative stress and hormonal milieu. Sera were assayed for inflammatory marker (C-reactive protein), oxidative stress and hormones. Animals with pyometra showed fever, polydipsia, polyuria, vomiting and purulent vulvar discharge. Ultrasound examination revealed the presence of anechoic to hypoechoic uterine fluid with an increased wall thickness. There was a significant increase in CRP, NO, MDA, P4, P4/E2 ratio and a decrease in SOD, CAT and TAC in bitches and queens. Histopathology showed a suppurative inflammation and heavy leucocytic infiltration in all uterine layers, and severe degenerative changes in endometrial glands with neutrophils-infiltrated homogeneous eosinophilic substances in the lumen. The ultrasound morphometric measurements (uterine diameter, wall thickness, and lumen and wall echo-pattern) were positively correlated with CRP and NO, and negatively correlated with TAC.
Chapter Ⅲ aimed to clarify the alterations in hormones, macro-element (calcium, phosphorus), alpha-fetoprotein and oxidative stress biomarkers. Dystocial animals showed hypothermia, anorexia, greenish vulvar discharge and strong abdominal contractions without fetal delivery. Ultrasound revealed weak fetal heart rate and some abnormal fetal dispositions. Radiography confirmed the abnormal fetal altitude and fetal number. There was a significant increase in P4, and a significant decrease in E2, Ca, SOD and CAT in dystocia suffered bitches and queens, respectively compared to normal parturate animals. Pleuriparous bitches had lower MDA levels compared to primiparous ones. Animals with dystocial duration more than 24 hours had higher MDA levels compared to those with duration less than 24 hours. Animals with fetal viability > 50% showed marked elevation in TAC and NO than those with weak fetal viability. Queens with high fetal viability > 50% showed significant decrease in SOD and GPx than those with weak fetal viability.