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Prof. Abdou Mahdy Mohamed Mahdy :: Publications: |
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| Title: | Establishment of the regeneration and transformation systems of F144 sugarcane cultivar. Pakistan Journal of Biotechnology 2 (1-2): 15-18. www.askzad.com |
| Authors: | Attia, O. Attia; A.M.M. Mahdy, M.E. Hafez, A.S. Sadik and Naglaa Abdellah. |
| Year: | 2005 |
| Keywords: | Sugarcane, microprojectile mediated method, plasmid pAB6, GUS and BAR genes. |
| Journal: | Pakistan Journal of Biotechnology |
| Volume: | 2 |
| Issue: | 1-2 |
| Pages: | 15-18 |
| Publisher: | Not Available |
| Local/International: | Local |
| Paper Link: | Not Available |
| Full paper | Abdou Mahdy Mohamed Mahdy_PAPER_30.pdf |
| Supplementary materials | Not Available |
| Abstract: |
Sugarcane is one of the most important (two sugar) crops for sugar production in Egypt. In this study, an experiment was conducted to establishment the regeneration system for sugarcane., F144 using leaf bases as a explants through somatic embryogenesis. The effect of 2,4-D, NAA, IAA and kinetin hormones on the sugarcane regeneration was studied. The microprojectile mediated method was used to transform plasmid pAB6 carrying the gus and bar genes as reporter and selectable markers respectively. The presence of the trans- genes was confirmed by PCR analysis through which amplified fragments with sizes of about 1800(gus) and 540 bp (bar) were detected in transgenics. GUS assay and leaf painting detect the expression of both gus and bar genes. |
