Background: Chlamydia trachomatis (C.trachomatis )is the most prevalent sexually
transmitted pathogen worldwide. It is common among sexually active young women.
Objectives: Evaluate the sensitivity and specificity of tissue culture followed by antigen
detection by immunoflurescencein comparison with nested PCR for diagnosis of genital
C. trachomatis infection. Methodology: This study was carried out on 50 women,
among those attending the Gynecology Outpatient Clinics of Benha University Hospital
during the period from May 2014 to March 2015. The participants suffer from symptoms
suggestive of genital C. trachomatis infection , their ages ranged from 20 to 39 years
old (mean ± SD =29.80±4.647) .Two endocervical swabs were taken from each patient .
One used for cytological examination, and the other used for tissue culture detected by ,
immunoflurescence as well as nested PCR. Results: The result of tissue culture detected
by direct immunoflurecence revealed that out of 50 patient , 24 (48%) were positive for
C.trachomatis and 26 (52%) were negative .Result of nested PCR revealed that out of
50 patient, 34 (68%) were positive and 24 (32%) were negative . The result of
cytological examination revealed that out of 50 patient , 17 (34%) were positive and 33
(66%) were negative. The sensitivity, specificity, Positive Predictive Value (PPV), and
Negative Predictive Value (NPP) of tissue culture for detection of C.trachomatis were
70.6%, 100%, 100% and 61.5% respectively. Roc curve of tissue culture revealed that
it is considered to be a good test compared to PCR in diagnosis of genital C.trachomatis.
Conclusion: The tissue culture as detected by immunoflurescence is a good test in
relation to nested PCR in diagnosis of genital C.trachomatis |
