A total number of 150 Hake fish; Saurus tumbil were collected from Suez Governorate, of
average body weight 250 ± 50g during the period from 2005 – 2008. Clinical picture,
parasitological and molecular biological studies were performed on the isolated spores.
Stained nodular contents from the examined Hake fish showed thick walled spores, pyriform
or egg shaped and composed of sporoplasm, single nucleus, polar tube with a large posterior
vacuole. Based on the morphological and parasitological findings, the isolated parasite was
related to Glugea sp. The total prevalence of microsporidiosis in wild Hake fish was 16.67%.
Seasonally, the spring season gave the suitable environmental conditions for such infection.
Molecular biological findings of the whole microsporidian nodule possessed several protein
bands reaching up to 14 bands of different molecular weights ranging from 31.421- 68.629
KDa. The average molecular weights of the revealed protein bands were 47.651 KDa. The
migration depth of the measured protein (absorbance through the gel) was pronounced by a
constructed diagram (curve) on the measuring unit. Polymerase Chain Reaction (PCR) of
genomic DNA recorded fingerprint of Hake Microsporidia against 13 random primers, in
which multiple bands were recorded ranging from 3-13 bands on each primer used. The
fingerprint of such microsporidia was recorded and kept for any further studies at the
Agriculture Genetic Engineering Institute, Agriculture Research Centre, Giza. |
