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Dr. Fouad Fouad Saad Elmayet :: Publications:

Title:
Molecular Detection and Phylogenetic Analyses to Evaluate the Evolutionary Pattern of N, P, and G Gene Sequences of Some Recent Rabies Virus (RABV) Isolates from Elwadi- Elgedid Province in Egypt.
Authors: Michael Salheen, Fouad S. El-mayet, Zeinab R. Aboezz, Ayman S. El-Habbaa, Marcus I.Yanni, Saad S.A. Sharawi
Year: 2021
Keywords: Molecular analysis, nucleoprotein (N), genes, Rabies, Elwadi- Elgedid.
Journal: Advances in Animal and Veterinary Sciences
Volume: 9
Issue: 11
Pages: 1989-1994
Publisher: Nexus
Local/International: International
Paper Link:
Full paper Fouad Fouad Saad Elmayet_Final Publication in journal.pdf
Supplementary materials Not Available
Abstract:

Rabies is a fatal zoonotic infection caused by negative-strand RNA-virus classified in the genus Lyssavirus, family Rhabdoviridae of the order Mononegavirales. Despite the importance of the RABV for human and animal health, little is known about the spread of the virus in different animal populations in Egypt. Therefore, the current study aimed to identify and analyze the nucleotide sequences of nucleoprotein (N), phosphoprotein (P) and Glycoprotein (G) genes of the rabies virus from 10 suspected rabid cases of cattle and foxes from Elwadi- Elgedid province, which collected in 2017-2019. Specimens from the brains of suspected animals with rabies were collected and identified using direct FAT and RT-PCR. Three positive samples were further subjected to sequencing the partially amplified N, P, and G genes. The phylogenetic analysis was performed concerning the Egyptian rabies virus isolates, vaccinal strains and other sequences from the neighbouring countries available on the GenBank. It was found that the nucleotide identity in comparison to our new Egyptian isolates of Elwadi- Elgedid decreases by time at the level of three genes of previously isolated strains. Our results revealed that these new RABV isolates were more homologous to each other and were genetically related to Egyptian dog rabies, which previously identified two decades ago. The studied vaccinal strains were of low nucleotide identity with the new isolates. They also share some similarities with the RABV isolates in the neighbouring endemic regions in the Middle East. Furthermore, these data extend our knowledge of the relatedness and genetic variation of RABVs circulating in Egypt.

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